Western Blot:Article Title: Tobacco-induced hyperglycemia promotes lung cancer progression via cancer cell-macrophage interaction through paracrine IGF2/IR/NPM1-driven PD-L1 expression
Article Snippet: .. Antibodies used in this study Target Vendor Catalogue Clone Dilution ratio Application CD45 BD Bioscience 550539 30-F11 1:200 IF F4/80 Bio-Rad MCA497 1:200 IF MPO Abcam 208670 1:200 IF α-SMA Sigma Aldrich A2547 1:200 IF CD4 Bio-Rad MCA1767T 1:200 IF CD8 Abcam 22378 1:200 IF CD206 Proteintech 18704-1-AP 1:1000 WB Arginase 1 Cell signaling 93668 D4E3M 1:200 (IF) 1:1000 (WB) IF, WB iNOS Abcam ab178945 1:200 IF Granzyme B R&D AF-1865 1:200 IF GLUT1 Novus Biologicals NB110-39113 1:500 (IF) 1:1000 (WB) IF, WB GLUT3 Proteintech 20403-1-AP 1:500 (IF) 1:1000 (WB) IF, WB NA+K+ATPase Cell signaling 3010 1:1000 WB pIGF-1R (Y1131) /IR (Y1146) Cell signaling 3021 1:100 (IHC) 1:200 (IF) IF, IHC pIGF-1R (Y1135/36) /IR (Y1150/1151) Cell signaling 3024 19H7 1:1000 WB IR Santa Cruz sc-711 C-19 1:200 CHIP IR Santa Cruz sc-57342 CT-3 1:200 (IP) 1:1000 (WB) IP, WB IR Cell signaling 3020 L55B10 1:1000 WB IGF-1R Cell signaling 3027 1:1000 WB IGF2 Abcam ab9574 1:1000 (WB) 1:200 (IF) WB IF NPM1 Abcam 10530 1:1000 WB NPM1 Santa Cruz sc-32256 1:200 IP, CHIP PD-L1 Cell signaling 13684 E1L3N 1:1000 WB OCT-A Santa Cruz sc-166355 H-5 1:1000 (WB) 1:200 (IP) WB, IP OCT-A Santa Cruz 807 D-8 1:1000 (WB) WB human CD274 BioLegend 329402 29E.2A3 1:100 IF mouse CD274 BioLegend 124302 10F.9G2 1:100 IHC - 5 - mouse PD-L1 BioXCell BE0101 10F.9G2 8 μg/mL (WB) 10 μg/mL (blockade) WB, Blockade TTF1 Santa Cruz sc-13040 1:100 IHC APC/Cy7 anti-mouse CD45 BioLegend 103116 30-F11 1:100 FACS PE/Cy7 anti-mouse F4/80 BioLegend 123114 BM8 1:100 FACS 1)Application - WB: Western blot analysis; IF: Immunofluorescence staining; IHC: Immunohistochemistry; IP: Immunoprecipitation; FACS: Fluorescence-Activated Cell Sorting, Supplementary Table 4. ..
Article Title: Macrophage Extracellular Traps Modulate the Compensatory Anti-inflammatory Response Syndrome through IL-33/ST2 Signaling in Severe Acute Pancreatitis
Article Snippet: Primary/Secondary antibody Company Cat. No Dilution NLRP3 CST 15101 1:1000 GSDMD Abcam ab219800 1:1000 N-GSDMD Affinity DF13758 1:1000 for WB, 1:500 for IHC AMYLASE CST 3796 1:1000 for WB, 1:500 for IHC ACTIN Proteintech 66009-1-Ig 1:1000 MPO Abcam ab208670 1:1000 for WB, 1:500 for IF H3CIT Abcam ab281584 1:1000 NE Abcam ab131260 1:1000 Caspase4 Proteintech 11856-1-AP 1:1000 Cleaved-Caspase-1 Affinity AF4005 1:1000 IL-33 (FOR WB) Abclonal A8096 1:1000 IL-33 (FOR IHC) Abways CY5105 1:100 ST2 Abcam ab194113 1:500 P-STAT6 Affinity AF3301 1:500 MMP12 Dreambio YM-Y23544 1:1000 LY6G Abclonal A22270 1:100 GATA3 Affinity AF6233 1:100 TBET Affinity DF7759 1:100 CD68 Abcam ab303565 1:500 CD68 Abcam ab53444 1:500 CD86 Abways DF6332 1:500 CD206 Proteintech 18704-1-AP 1:500 TBK1 Abways CY5145 1:1000 P-TBK1 Abways CY8364 1:1000 STING Proteintech 19851-1-AP 1:1000 P-STING Affinity AF7416 1:1000 IRF3 Abways CY5779 1:1000 P-IRF3 Proteintech 29528-1-AP 1:1000 FITC Anti-Mouse CD4 Antibody[RM4-5] Elabscience E-AB-F1353C 1:20 .. Primary/Secondary antibody Company Cat. No Dilution NLRP3 CST 15101 1:1000 GSDMD Abcam ab219800 1:1000 N-GSDMD Affinity DF13758 1:1000 for WB, 1:500 for IHC AMYLASE CST 3796 1:1000 for WB, 1:500 for IHC ACTIN Proteintech 66009-1-Ig 1:1000 MPO Abcam ab208670 1:1000 for WB, 1:500 for IF H3CIT Abcam ab281584 1:1000 NE Abcam ab131260 1:1000 Caspase4 Proteintech 11856-1-AP 1:1000 Cleaved-Caspase-1 Affinity AF4005 1:1000 IL-33 (FOR WB) Abclonal A8096 1:1000 IL-33 (FOR IHC) Abways CY5105 1:100 ST2 Abcam ab194113 1:500 P-STAT6 Affinity AF3301 1:500 MMP12 Dreambio YM-Y23544 1:1000 LY6G Abclonal A22270 1:100 GATA3 Affinity AF6233 1:100 TBET Affinity DF7759 1:100 CD68 Abcam ab303565 1:500 CD68 Abcam ab53444 1:500 CD86 Abways DF6332 1:500 CD206 Proteintech 18704-1-AP 1:500 TBK1 Abways CY5145 1:1000 P-TBK1 Abways CY8364 1:1000 STING Proteintech 19851-1-AP 1:1000 P-STING Affinity AF7416 1:1000 IRF3 Abways CY5779 1:1000 P-IRF3 Proteintech 29528-1-AP 1:1000 FITC Anti-Mouse CD4 Antibody[RM4-5] Elabscience E-AB-F1353C 1:20 APC Anti-Mouse Foxp3 Antibody[3G3] Elabscience E-AB-F1238E 1:20 APC Anti-Mouse IL-4 Antibody[11B11] Elabscience E-AB-F1204E 1:20 PE Anti-Mouse F4/80 Elabscience E-AB-F0995D 1:20 Antibody[CI:A3-1] APC Anti-Mouse CD206/MMR Antibody[C068C2] Elabscience E-AB-F1135E 1:20 PE Anti-Mouse IFN-γ Antibody[XMG1.2] Elabscience E-AB-F1101D 1:20 Alexa Fluor 488-labeled Goat Anti-Rabbit IgG(H+L) Beyotime A0423 1:200 Alexa Fluor 488-labeled Goat Anti-Mouse IgG(H+L) Beyotime A0473 1:200 Cy3-labeled Goat Anti-Rat IgG(H+L) Beyotime A0507 1:200 Cy3-labeled Goat Anti-Rabbit IgG (H+L) Beyotime A0562 1:200 FITC-labeled Goat Anti-Mouse IgG (H+L) Beyotime A0568 1:200 Anti-rabbit IgG, HRP-linked Antibody CST 7074 1:5000 Anti-mouse IgG, HRP-linked Antibody CST 7076 1:5000 HRP-labeled Goat Anti-Rabbit IgG(H+L) Beyotime A0208 1:200 HRP-labeled Rabbit Anti-Mouse IgG(H+L) Beyotime A0354 1:200 ..
Article Title: Macrophage Extracellular Traps Modulate the Compensatory Anti-inflammatory Response Syndrome through IL-33/ST2 Signaling in Severe Acute Pancreatitis
Article Snippet: .. Primary/Secondary antibody Company Cat. No Dilution NLRP3 CST 15101 1:1000 GSDMD Abcam ab219800 1:1000 N-GSDMD Affinity DF13758 1:1000 for WB, 1:500 for IHC AMYLASE CST 3796 1:1000 for WB, 1:500 for IHC ACTIN Proteintech 66009-1-Ig 1:1000 MPO Abcam ab208670 1:1000 for WB, 1:500 for IF H3CIT Abcam ab281584 1:1000 NE Abcam ab131260 1:1000 Caspase4 Proteintech 11856-1-AP 1:1000 Cleaved-Caspase-1 Affinity AF4005 1:1000 IL-33 (FOR WB) Abclonal A8096 1:1000 IL-33 (FOR IHC) Abways CY5105 1:100 ST2 Abcam ab194113 1:500 P-STAT6 Affinity AF3301 1:500 MMP12 Dreambio YM-Y23544 1:1000 LY6G Abclonal A22270 1:100 GATA3 Affinity AF6233 1:100 TBET Affinity DF7759 1:100 CD68 Abcam ab303565 1:500 CD68 Abcam ab53444 1:500 CD86 Abways DF6332 1:500 CD206 Proteintech 18704-1-AP 1:500 TBK1 Abways CY5145 1:1000 P-TBK1 Abways CY8364 1:1000 STING Proteintech 19851-1-AP 1:1000 P-STING Affinity AF7416 1:1000 IRF3 Abways CY5779 1:1000 P-IRF3 Proteintech 29528-1-AP 1:1000 FITC Anti-Mouse CD4 Antibody[RM4-5] Elabscience E-AB-F1353C 1:20 .. Primary/Secondary antibody Company Cat. No Dilution NLRP3 CST 15101 1:1000 GSDMD Abcam ab219800 1:1000 N-GSDMD Affinity DF13758 1:1000 for WB, 1:500 for IHC AMYLASE CST 3796 1:1000 for WB, 1:500 for IHC ACTIN Proteintech 66009-1-Ig 1:1000 MPO Abcam ab208670 1:1000 for WB, 1:500 for IF H3CIT Abcam ab281584 1:1000 NE Abcam ab131260 1:1000 Caspase4 Proteintech 11856-1-AP 1:1000 Cleaved-Caspase-1 Affinity AF4005 1:1000 IL-33 (FOR WB) Abclonal A8096 1:1000 IL-33 (FOR IHC) Abways CY5105 1:100 ST2 Abcam ab194113 1:500 P-STAT6 Affinity AF3301 1:500 MMP12 Dreambio YM-Y23544 1:1000 LY6G Abclonal A22270 1:100 GATA3 Affinity AF6233 1:100 TBET Affinity DF7759 1:100 CD68 Abcam ab303565 1:500 CD68 Abcam ab53444 1:500 CD86 Abways DF6332 1:500 CD206 Proteintech 18704-1-AP 1:500 TBK1 Abways CY5145 1:1000 P-TBK1 Abways CY8364 1:1000 STING Proteintech 19851-1-AP 1:1000 P-STING Affinity AF7416 1:1000 IRF3 Abways CY5779 1:1000 P-IRF3 Proteintech 29528-1-AP 1:1000 FITC Anti-Mouse CD4 Antibody[RM4-5] Elabscience E-AB-F1353C 1:20 APC Anti-Mouse Foxp3 Antibody[3G3] Elabscience E-AB-F1238E 1:20 APC Anti-Mouse IL-4 Antibody[11B11] Elabscience E-AB-F1204E 1:20 PE Anti-Mouse F4/80 Elabscience E-AB-F0995D 1:20 Antibody[CI:A3-1] APC Anti-Mouse CD206/MMR Antibody[C068C2] Elabscience E-AB-F1135E 1:20 PE Anti-Mouse IFN-γ Antibody[XMG1.2] Elabscience E-AB-F1101D 1:20 Alexa Fluor 488-labeled Goat Anti-Rabbit IgG(H+L) Beyotime A0423 1:200 Alexa Fluor 488-labeled Goat Anti-Mouse IgG(H+L) Beyotime A0473 1:200 Cy3-labeled Goat Anti-Rat IgG(H+L) Beyotime A0507 1:200 Cy3-labeled Goat Anti-Rabbit IgG (H+L) Beyotime A0562 1:200 FITC-labeled Goat Anti-Mouse IgG (H+L) Beyotime A0568 1:200 Anti-rabbit IgG, HRP-linked Antibody CST 7074 1:5000 Anti-mouse IgG, HRP-linked Antibody CST 7076 1:5000 HRP-labeled Goat Anti-Rabbit IgG(H+L) Beyotime A0208 1:200 HRP-labeled Rabbit Anti-Mouse IgG(H+L) Beyotime A0354 1:200
Immunohistochemistry:Article Title: Tobacco-induced hyperglycemia promotes lung cancer progression via cancer cell-macrophage interaction through paracrine IGF2/IR/NPM1-driven PD-L1 expression
Article Snippet: .. Antibodies used in this study Target Vendor Catalogue Clone Dilution ratio Application CD45 BD Bioscience 550539 30-F11 1:200 IF F4/80 Bio-Rad MCA497 1:200 IF MPO Abcam 208670 1:200 IF α-SMA Sigma Aldrich A2547 1:200 IF CD4 Bio-Rad MCA1767T 1:200 IF CD8 Abcam 22378 1:200 IF CD206 Proteintech 18704-1-AP 1:1000 WB Arginase 1 Cell signaling 93668 D4E3M 1:200 (IF) 1:1000 (WB) IF, WB iNOS Abcam ab178945 1:200 IF Granzyme B R&D AF-1865 1:200 IF GLUT1 Novus Biologicals NB110-39113 1:500 (IF) 1:1000 (WB) IF, WB GLUT3 Proteintech 20403-1-AP 1:500 (IF) 1:1000 (WB) IF, WB NA+K+ATPase Cell signaling 3010 1:1000 WB pIGF-1R (Y1131) /IR (Y1146) Cell signaling 3021 1:100 (IHC) 1:200 (IF) IF, IHC pIGF-1R (Y1135/36) /IR (Y1150/1151) Cell signaling 3024 19H7 1:1000 WB IR Santa Cruz sc-711 C-19 1:200 CHIP IR Santa Cruz sc-57342 CT-3 1:200 (IP) 1:1000 (WB) IP, WB IR Cell signaling 3020 L55B10 1:1000 WB IGF-1R Cell signaling 3027 1:1000 WB IGF2 Abcam ab9574 1:1000 (WB) 1:200 (IF) WB IF NPM1 Abcam 10530 1:1000 WB NPM1 Santa Cruz sc-32256 1:200 IP, CHIP PD-L1 Cell signaling 13684 E1L3N 1:1000 WB OCT-A Santa Cruz sc-166355 H-5 1:1000 (WB) 1:200 (IP) WB, IP OCT-A Santa Cruz 807 D-8 1:1000 (WB) WB human CD274 BioLegend 329402 29E.2A3 1:100 IF mouse CD274 BioLegend 124302 10F.9G2 1:100 IHC - 5 - mouse PD-L1 BioXCell BE0101 10F.9G2 8 μg/mL (WB) 10 μg/mL (blockade) WB, Blockade TTF1 Santa Cruz sc-13040 1:100 IHC APC/Cy7 anti-mouse CD45 BioLegend 103116 30-F11 1:100 FACS PE/Cy7 anti-mouse F4/80 BioLegend 123114 BM8 1:100 FACS 1)Application - WB: Western blot analysis; IF: Immunofluorescence staining; IHC: Immunohistochemistry; IP: Immunoprecipitation; FACS: Fluorescence-Activated Cell Sorting, Supplementary Table 4. ..
Article Title: Macrophage Extracellular Traps Modulate the Compensatory Anti-inflammatory Response Syndrome through IL-33/ST2 Signaling in Severe Acute Pancreatitis
Article Snippet: Primary/Secondary antibody Company Cat. No Dilution NLRP3 CST 15101 1:1000 GSDMD Abcam ab219800 1:1000 N-GSDMD Affinity DF13758 1:1000 for WB, 1:500 for IHC AMYLASE CST 3796 1:1000 for WB, 1:500 for IHC ACTIN Proteintech 66009-1-Ig 1:1000 MPO Abcam ab208670 1:1000 for WB, 1:500 for IF H3CIT Abcam ab281584 1:1000 NE Abcam ab131260 1:1000 Caspase4 Proteintech 11856-1-AP 1:1000 Cleaved-Caspase-1 Affinity AF4005 1:1000 IL-33 (FOR WB) Abclonal A8096 1:1000 IL-33 (FOR IHC) Abways CY5105 1:100 ST2 Abcam ab194113 1:500 P-STAT6 Affinity AF3301 1:500 MMP12 Dreambio YM-Y23544 1:1000 LY6G Abclonal A22270 1:100 GATA3 Affinity AF6233 1:100 TBET Affinity DF7759 1:100 CD68 Abcam ab303565 1:500 CD68 Abcam ab53444 1:500 CD86 Abways DF6332 1:500 CD206 Proteintech 18704-1-AP 1:500 TBK1 Abways CY5145 1:1000 P-TBK1 Abways CY8364 1:1000 STING Proteintech 19851-1-AP 1:1000 P-STING Affinity AF7416 1:1000 IRF3 Abways CY5779 1:1000 P-IRF3 Proteintech 29528-1-AP 1:1000 FITC Anti-Mouse CD4 Antibody[RM4-5] Elabscience E-AB-F1353C 1:20 .. Primary/Secondary antibody Company Cat. No Dilution NLRP3 CST 15101 1:1000 GSDMD Abcam ab219800 1:1000 N-GSDMD Affinity DF13758 1:1000 for WB, 1:500 for IHC AMYLASE CST 3796 1:1000 for WB, 1:500 for IHC ACTIN Proteintech 66009-1-Ig 1:1000 MPO Abcam ab208670 1:1000 for WB, 1:500 for IF H3CIT Abcam ab281584 1:1000 NE Abcam ab131260 1:1000 Caspase4 Proteintech 11856-1-AP 1:1000 Cleaved-Caspase-1 Affinity AF4005 1:1000 IL-33 (FOR WB) Abclonal A8096 1:1000 IL-33 (FOR IHC) Abways CY5105 1:100 ST2 Abcam ab194113 1:500 P-STAT6 Affinity AF3301 1:500 MMP12 Dreambio YM-Y23544 1:1000 LY6G Abclonal A22270 1:100 GATA3 Affinity AF6233 1:100 TBET Affinity DF7759 1:100 CD68 Abcam ab303565 1:500 CD68 Abcam ab53444 1:500 CD86 Abways DF6332 1:500 CD206 Proteintech 18704-1-AP 1:500 TBK1 Abways CY5145 1:1000 P-TBK1 Abways CY8364 1:1000 STING Proteintech 19851-1-AP 1:1000 P-STING Affinity AF7416 1:1000 IRF3 Abways CY5779 1:1000 P-IRF3 Proteintech 29528-1-AP 1:1000 FITC Anti-Mouse CD4 Antibody[RM4-5] Elabscience E-AB-F1353C 1:20 APC Anti-Mouse Foxp3 Antibody[3G3] Elabscience E-AB-F1238E 1:20 APC Anti-Mouse IL-4 Antibody[11B11] Elabscience E-AB-F1204E 1:20 PE Anti-Mouse F4/80 Elabscience E-AB-F0995D 1:20 Antibody[CI:A3-1] APC Anti-Mouse CD206/MMR Antibody[C068C2] Elabscience E-AB-F1135E 1:20 PE Anti-Mouse IFN-γ Antibody[XMG1.2] Elabscience E-AB-F1101D 1:20 Alexa Fluor 488-labeled Goat Anti-Rabbit IgG(H+L) Beyotime A0423 1:200 Alexa Fluor 488-labeled Goat Anti-Mouse IgG(H+L) Beyotime A0473 1:200 Cy3-labeled Goat Anti-Rat IgG(H+L) Beyotime A0507 1:200 Cy3-labeled Goat Anti-Rabbit IgG (H+L) Beyotime A0562 1:200 FITC-labeled Goat Anti-Mouse IgG (H+L) Beyotime A0568 1:200 Anti-rabbit IgG, HRP-linked Antibody CST 7074 1:5000 Anti-mouse IgG, HRP-linked Antibody CST 7076 1:5000 HRP-labeled Goat Anti-Rabbit IgG(H+L) Beyotime A0208 1:200 HRP-labeled Rabbit Anti-Mouse IgG(H+L) Beyotime A0354 1:200 ..
Article Title: Macrophage Extracellular Traps Modulate the Compensatory Anti-inflammatory Response Syndrome through IL-33/ST2 Signaling in Severe Acute Pancreatitis
Article Snippet: .. Primary/Secondary antibody Company Cat. No Dilution NLRP3 CST 15101 1:1000 GSDMD Abcam ab219800 1:1000 N-GSDMD Affinity DF13758 1:1000 for WB, 1:500 for IHC AMYLASE CST 3796 1:1000 for WB, 1:500 for IHC ACTIN Proteintech 66009-1-Ig 1:1000 MPO Abcam ab208670 1:1000 for WB, 1:500 for IF H3CIT Abcam ab281584 1:1000 NE Abcam ab131260 1:1000 Caspase4 Proteintech 11856-1-AP 1:1000 Cleaved-Caspase-1 Affinity AF4005 1:1000 IL-33 (FOR WB) Abclonal A8096 1:1000 IL-33 (FOR IHC) Abways CY5105 1:100 ST2 Abcam ab194113 1:500 P-STAT6 Affinity AF3301 1:500 MMP12 Dreambio YM-Y23544 1:1000 LY6G Abclonal A22270 1:100 GATA3 Affinity AF6233 1:100 TBET Affinity DF7759 1:100 CD68 Abcam ab303565 1:500 CD68 Abcam ab53444 1:500 CD86 Abways DF6332 1:500 CD206 Proteintech 18704-1-AP 1:500 TBK1 Abways CY5145 1:1000 P-TBK1 Abways CY8364 1:1000 STING Proteintech 19851-1-AP 1:1000 P-STING Affinity AF7416 1:1000 IRF3 Abways CY5779 1:1000 P-IRF3 Proteintech 29528-1-AP 1:1000 FITC Anti-Mouse CD4 Antibody[RM4-5] Elabscience E-AB-F1353C 1:20 .. Primary/Secondary antibody Company Cat. No Dilution NLRP3 CST 15101 1:1000 GSDMD Abcam ab219800 1:1000 N-GSDMD Affinity DF13758 1:1000 for WB, 1:500 for IHC AMYLASE CST 3796 1:1000 for WB, 1:500 for IHC ACTIN Proteintech 66009-1-Ig 1:1000 MPO Abcam ab208670 1:1000 for WB, 1:500 for IF H3CIT Abcam ab281584 1:1000 NE Abcam ab131260 1:1000 Caspase4 Proteintech 11856-1-AP 1:1000 Cleaved-Caspase-1 Affinity AF4005 1:1000 IL-33 (FOR WB) Abclonal A8096 1:1000 IL-33 (FOR IHC) Abways CY5105 1:100 ST2 Abcam ab194113 1:500 P-STAT6 Affinity AF3301 1:500 MMP12 Dreambio YM-Y23544 1:1000 LY6G Abclonal A22270 1:100 GATA3 Affinity AF6233 1:100 TBET Affinity DF7759 1:100 CD68 Abcam ab303565 1:500 CD68 Abcam ab53444 1:500 CD86 Abways DF6332 1:500 CD206 Proteintech 18704-1-AP 1:500 TBK1 Abways CY5145 1:1000 P-TBK1 Abways CY8364 1:1000 STING Proteintech 19851-1-AP 1:1000 P-STING Affinity AF7416 1:1000 IRF3 Abways CY5779 1:1000 P-IRF3 Proteintech 29528-1-AP 1:1000 FITC Anti-Mouse CD4 Antibody[RM4-5] Elabscience E-AB-F1353C 1:20 APC Anti-Mouse Foxp3 Antibody[3G3] Elabscience E-AB-F1238E 1:20 APC Anti-Mouse IL-4 Antibody[11B11] Elabscience E-AB-F1204E 1:20 PE Anti-Mouse F4/80 Elabscience E-AB-F0995D 1:20 Antibody[CI:A3-1] APC Anti-Mouse CD206/MMR Antibody[C068C2] Elabscience E-AB-F1135E 1:20 PE Anti-Mouse IFN-γ Antibody[XMG1.2] Elabscience E-AB-F1101D 1:20 Alexa Fluor 488-labeled Goat Anti-Rabbit IgG(H+L) Beyotime A0423 1:200 Alexa Fluor 488-labeled Goat Anti-Mouse IgG(H+L) Beyotime A0473 1:200 Cy3-labeled Goat Anti-Rat IgG(H+L) Beyotime A0507 1:200 Cy3-labeled Goat Anti-Rabbit IgG (H+L) Beyotime A0562 1:200 FITC-labeled Goat Anti-Mouse IgG (H+L) Beyotime A0568 1:200 Anti-rabbit IgG, HRP-linked Antibody CST 7074 1:5000 Anti-mouse IgG, HRP-linked Antibody CST 7076 1:5000 HRP-labeled Goat Anti-Rabbit IgG(H+L) Beyotime A0208 1:200 HRP-labeled Rabbit Anti-Mouse IgG(H+L) Beyotime A0354 1:200
FACS:Article Title: Tobacco-induced hyperglycemia promotes lung cancer progression via cancer cell-macrophage interaction through paracrine IGF2/IR/NPM1-driven PD-L1 expression
Article Snippet: .. Antibodies used in this study Target Vendor Catalogue Clone Dilution ratio Application CD45 BD Bioscience 550539 30-F11 1:200 IF F4/80 Bio-Rad MCA497 1:200 IF MPO Abcam 208670 1:200 IF α-SMA Sigma Aldrich A2547 1:200 IF CD4 Bio-Rad MCA1767T 1:200 IF CD8 Abcam 22378 1:200 IF CD206 Proteintech 18704-1-AP 1:1000 WB Arginase 1 Cell signaling 93668 D4E3M 1:200 (IF) 1:1000 (WB) IF, WB iNOS Abcam ab178945 1:200 IF Granzyme B R&D AF-1865 1:200 IF GLUT1 Novus Biologicals NB110-39113 1:500 (IF) 1:1000 (WB) IF, WB GLUT3 Proteintech 20403-1-AP 1:500 (IF) 1:1000 (WB) IF, WB NA+K+ATPase Cell signaling 3010 1:1000 WB pIGF-1R (Y1131) /IR (Y1146) Cell signaling 3021 1:100 (IHC) 1:200 (IF) IF, IHC pIGF-1R (Y1135/36) /IR (Y1150/1151) Cell signaling 3024 19H7 1:1000 WB IR Santa Cruz sc-711 C-19 1:200 CHIP IR Santa Cruz sc-57342 CT-3 1:200 (IP) 1:1000 (WB) IP, WB IR Cell signaling 3020 L55B10 1:1000 WB IGF-1R Cell signaling 3027 1:1000 WB IGF2 Abcam ab9574 1:1000 (WB) 1:200 (IF) WB IF NPM1 Abcam 10530 1:1000 WB NPM1 Santa Cruz sc-32256 1:200 IP, CHIP PD-L1 Cell signaling 13684 E1L3N 1:1000 WB OCT-A Santa Cruz sc-166355 H-5 1:1000 (WB) 1:200 (IP) WB, IP OCT-A Santa Cruz 807 D-8 1:1000 (WB) WB human CD274 BioLegend 329402 29E.2A3 1:100 IF mouse CD274 BioLegend 124302 10F.9G2 1:100 IHC - 5 - mouse PD-L1 BioXCell BE0101 10F.9G2 8 μg/mL (WB) 10 μg/mL (blockade) WB, Blockade TTF1 Santa Cruz sc-13040 1:100 IHC APC/Cy7 anti-mouse CD45 BioLegend 103116 30-F11 1:100 FACS PE/Cy7 anti-mouse F4/80 BioLegend 123114 BM8 1:100 FACS 1)Application - WB: Western blot analysis; IF: Immunofluorescence staining; IHC: Immunohistochemistry; IP: Immunoprecipitation; FACS: Fluorescence-Activated Cell Sorting, Supplementary Table 4. ..
Immunofluorescence:Article Title: Tobacco-induced hyperglycemia promotes lung cancer progression via cancer cell-macrophage interaction through paracrine IGF2/IR/NPM1-driven PD-L1 expression
Article Snippet: .. Antibodies used in this study Target Vendor Catalogue Clone Dilution ratio Application CD45 BD Bioscience 550539 30-F11 1:200 IF F4/80 Bio-Rad MCA497 1:200 IF MPO Abcam 208670 1:200 IF α-SMA Sigma Aldrich A2547 1:200 IF CD4 Bio-Rad MCA1767T 1:200 IF CD8 Abcam 22378 1:200 IF CD206 Proteintech 18704-1-AP 1:1000 WB Arginase 1 Cell signaling 93668 D4E3M 1:200 (IF) 1:1000 (WB) IF, WB iNOS Abcam ab178945 1:200 IF Granzyme B R&D AF-1865 1:200 IF GLUT1 Novus Biologicals NB110-39113 1:500 (IF) 1:1000 (WB) IF, WB GLUT3 Proteintech 20403-1-AP 1:500 (IF) 1:1000 (WB) IF, WB NA+K+ATPase Cell signaling 3010 1:1000 WB pIGF-1R (Y1131) /IR (Y1146) Cell signaling 3021 1:100 (IHC) 1:200 (IF) IF, IHC pIGF-1R (Y1135/36) /IR (Y1150/1151) Cell signaling 3024 19H7 1:1000 WB IR Santa Cruz sc-711 C-19 1:200 CHIP IR Santa Cruz sc-57342 CT-3 1:200 (IP) 1:1000 (WB) IP, WB IR Cell signaling 3020 L55B10 1:1000 WB IGF-1R Cell signaling 3027 1:1000 WB IGF2 Abcam ab9574 1:1000 (WB) 1:200 (IF) WB IF NPM1 Abcam 10530 1:1000 WB NPM1 Santa Cruz sc-32256 1:200 IP, CHIP PD-L1 Cell signaling 13684 E1L3N 1:1000 WB OCT-A Santa Cruz sc-166355 H-5 1:1000 (WB) 1:200 (IP) WB, IP OCT-A Santa Cruz 807 D-8 1:1000 (WB) WB human CD274 BioLegend 329402 29E.2A3 1:100 IF mouse CD274 BioLegend 124302 10F.9G2 1:100 IHC - 5 - mouse PD-L1 BioXCell BE0101 10F.9G2 8 μg/mL (WB) 10 μg/mL (blockade) WB, Blockade TTF1 Santa Cruz sc-13040 1:100 IHC APC/Cy7 anti-mouse CD45 BioLegend 103116 30-F11 1:100 FACS PE/Cy7 anti-mouse F4/80 BioLegend 123114 BM8 1:100 FACS 1)Application - WB: Western blot analysis; IF: Immunofluorescence staining; IHC: Immunohistochemistry; IP: Immunoprecipitation; FACS: Fluorescence-Activated Cell Sorting, Supplementary Table 4. ..
Staining:Article Title: Tobacco-induced hyperglycemia promotes lung cancer progression via cancer cell-macrophage interaction through paracrine IGF2/IR/NPM1-driven PD-L1 expression
Article Snippet: .. Antibodies used in this study Target Vendor Catalogue Clone Dilution ratio Application CD45 BD Bioscience 550539 30-F11 1:200 IF F4/80 Bio-Rad MCA497 1:200 IF MPO Abcam 208670 1:200 IF α-SMA Sigma Aldrich A2547 1:200 IF CD4 Bio-Rad MCA1767T 1:200 IF CD8 Abcam 22378 1:200 IF CD206 Proteintech 18704-1-AP 1:1000 WB Arginase 1 Cell signaling 93668 D4E3M 1:200 (IF) 1:1000 (WB) IF, WB iNOS Abcam ab178945 1:200 IF Granzyme B R&D AF-1865 1:200 IF GLUT1 Novus Biologicals NB110-39113 1:500 (IF) 1:1000 (WB) IF, WB GLUT3 Proteintech 20403-1-AP 1:500 (IF) 1:1000 (WB) IF, WB NA+K+ATPase Cell signaling 3010 1:1000 WB pIGF-1R (Y1131) /IR (Y1146) Cell signaling 3021 1:100 (IHC) 1:200 (IF) IF, IHC pIGF-1R (Y1135/36) /IR (Y1150/1151) Cell signaling 3024 19H7 1:1000 WB IR Santa Cruz sc-711 C-19 1:200 CHIP IR Santa Cruz sc-57342 CT-3 1:200 (IP) 1:1000 (WB) IP, WB IR Cell signaling 3020 L55B10 1:1000 WB IGF-1R Cell signaling 3027 1:1000 WB IGF2 Abcam ab9574 1:1000 (WB) 1:200 (IF) WB IF NPM1 Abcam 10530 1:1000 WB NPM1 Santa Cruz sc-32256 1:200 IP, CHIP PD-L1 Cell signaling 13684 E1L3N 1:1000 WB OCT-A Santa Cruz sc-166355 H-5 1:1000 (WB) 1:200 (IP) WB, IP OCT-A Santa Cruz 807 D-8 1:1000 (WB) WB human CD274 BioLegend 329402 29E.2A3 1:100 IF mouse CD274 BioLegend 124302 10F.9G2 1:100 IHC - 5 - mouse PD-L1 BioXCell BE0101 10F.9G2 8 μg/mL (WB) 10 μg/mL (blockade) WB, Blockade TTF1 Santa Cruz sc-13040 1:100 IHC APC/Cy7 anti-mouse CD45 BioLegend 103116 30-F11 1:100 FACS PE/Cy7 anti-mouse F4/80 BioLegend 123114 BM8 1:100 FACS 1)Application - WB: Western blot analysis; IF: Immunofluorescence staining; IHC: Immunohistochemistry; IP: Immunoprecipitation; FACS: Fluorescence-Activated Cell Sorting, Supplementary Table 4. ..
Immunoprecipitation:Article Title: Tobacco-induced hyperglycemia promotes lung cancer progression via cancer cell-macrophage interaction through paracrine IGF2/IR/NPM1-driven PD-L1 expression
Article Snippet: .. Antibodies used in this study Target Vendor Catalogue Clone Dilution ratio Application CD45 BD Bioscience 550539 30-F11 1:200 IF F4/80 Bio-Rad MCA497 1:200 IF MPO Abcam 208670 1:200 IF α-SMA Sigma Aldrich A2547 1:200 IF CD4 Bio-Rad MCA1767T 1:200 IF CD8 Abcam 22378 1:200 IF CD206 Proteintech 18704-1-AP 1:1000 WB Arginase 1 Cell signaling 93668 D4E3M 1:200 (IF) 1:1000 (WB) IF, WB iNOS Abcam ab178945 1:200 IF Granzyme B R&D AF-1865 1:200 IF GLUT1 Novus Biologicals NB110-39113 1:500 (IF) 1:1000 (WB) IF, WB GLUT3 Proteintech 20403-1-AP 1:500 (IF) 1:1000 (WB) IF, WB NA+K+ATPase Cell signaling 3010 1:1000 WB pIGF-1R (Y1131) /IR (Y1146) Cell signaling 3021 1:100 (IHC) 1:200 (IF) IF, IHC pIGF-1R (Y1135/36) /IR (Y1150/1151) Cell signaling 3024 19H7 1:1000 WB IR Santa Cruz sc-711 C-19 1:200 CHIP IR Santa Cruz sc-57342 CT-3 1:200 (IP) 1:1000 (WB) IP, WB IR Cell signaling 3020 L55B10 1:1000 WB IGF-1R Cell signaling 3027 1:1000 WB IGF2 Abcam ab9574 1:1000 (WB) 1:200 (IF) WB IF NPM1 Abcam 10530 1:1000 WB NPM1 Santa Cruz sc-32256 1:200 IP, CHIP PD-L1 Cell signaling 13684 E1L3N 1:1000 WB OCT-A Santa Cruz sc-166355 H-5 1:1000 (WB) 1:200 (IP) WB, IP OCT-A Santa Cruz 807 D-8 1:1000 (WB) WB human CD274 BioLegend 329402 29E.2A3 1:100 IF mouse CD274 BioLegend 124302 10F.9G2 1:100 IHC - 5 - mouse PD-L1 BioXCell BE0101 10F.9G2 8 μg/mL (WB) 10 μg/mL (blockade) WB, Blockade TTF1 Santa Cruz sc-13040 1:100 IHC APC/Cy7 anti-mouse CD45 BioLegend 103116 30-F11 1:100 FACS PE/Cy7 anti-mouse F4/80 BioLegend 123114 BM8 1:100 FACS 1)Application - WB: Western blot analysis; IF: Immunofluorescence staining; IHC: Immunohistochemistry; IP: Immunoprecipitation; FACS: Fluorescence-Activated Cell Sorting, Supplementary Table 4. ..
other:Article Title: Lactate transmission from hypoxic tumor cells promotes macrophage senescence and M2 polarization via the DNMT1-NHE7 axis to accelerate endometrial cancer progression.
Article Snippet: To establish NHE7-overexpressing and DNMT1-overexpressing M0 macrophages, as well as HIF1A-overexpressing Ishikawa cells, plasmids encoding NHE7 (NM_001257291), DNMT1 (NM_001130823), HIF1A (NM_001530), Name Brand Number Dilution rate Applications HIF1A proteintech 20960-1-AP 1:5000/1:200 WB/ IHC LDHA proteintech 21799-1-AP 1:5000/1:200 WB/IHC ENO2 proteintech 66150-1-Ig 1:5000/1:1000 WB/IHC MCT3 NOVUS NBP1-59885 1 μg/ml/1:200 WB/IHC HK2 proteintech 22029-1-AP 1:2000/1:1000 WB/IHC PGK1 proteintech 68035-1-Ig 1:10000/1:1000 WB/IHC ALDOB proteintech 18065-1-AP 1:10000/1:500 WB/IHC β-actin CST 4970 T 1:1000 WB H3K18la PTM BIO PTM-1427RM 1:1000/1:50 WB/CHIP H3 CST 14269S 1:1000 WB SLC9A7 Thermo PA5-106748 1:1000/1:200 WB/IF SLC9A7 Elabscience E-AB-12875 1:200 IHC Flag CST 14793S 1:1000 WB p53 proteintech 21891-1-AP 1:2000 WB p21 abcam ab109520 1:5000/1:100 WB/IF p16 Santa Cruz sc-1661 1:1000/1:200 WB/IF p-ERK CST 4370 s 1:2000 WB ERK CST 4695 s 1:1000 WB p-MEK CST 9154 T 1:1000 WB MEK proteintech 11049-1-AP 1:20000 WB DNMT1 CST 5032 T 1:1000/1:100 WB/ IF CD68 proteintech 25747-1-AP 1:5000 IF CD206 proteintech 18704-1-AP 1:500 IF Ki67 Abclonal A20018 1:500 IHC Anti-rabbit IgG, HRP-linked Antibody CST 7074P2 1: 2000 WB Anti-mouse IgG, HRP-linked Antibody CST 7076P2 1: 2000 WB Anti-rabbit IgG Antibody Servicebio G1213 1:200 IHC Tri-color Multiple Immunofluorescence Kit Shanghai Recordbio RC0086-23RM 1: 200 IF CD163 Invitrogen 17-1639-42 5 μL(0.5 μg)/test Flow cytometry CD68 Invitrogen 12-0689-42 5 μL (0.5 μg)/test Flow cytometry Cell Death and Disease (2026) 17:185 and the empty pCDH vector (VT1480) were procured from Youbio (Hunan, China).
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